Cryo-EM structure of a respiratory complex I mutant lacking NDUFS4
Resolution: 4.04 Å
EM Method: Single-particle
Fitted PDBs: 6rfs
Q-score: 0.342
Parey K, Haapanen O, Sharma V, Kofeler H, Zullig T, Prinz S, Siegmund K, Wittig I, Mills DJ, Vonck J, Kuhlbrandt W, Zickermann V
Sci Adv (2019) 5 pp. eaax9484-eaax9484 [ DOI: doi:10.1126/sciadv.aax9484 Pubmed: 31844670 ]
- Subunit niam of nadh:ubiquinone oxidoreductase (complex i) (17 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein acpm1 of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Subunit nb6m of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit nuem of nadh:ubiquinone oxidoreductase (complex i) (42 kDa, Protein from Yarrowia lipolytica)
- Subunit n7bm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nuam of nadh:ubiquinone oxidoreductase (complex i) (79 kDa, Protein from Candida lipolytica)
- Subunit nb8m of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
- Subunit nuvm of nadh:ubiquinone oxidoreductase (complex i) (7 kDa, Protein from Yarrowia lipolytica)
- Subunit nimm of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Subunit nuxm of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Candida lipolytica)
- Subunit nu1m of nadh:ubiquinone oxidoreductase (complex i) (38 kDa, Protein from Yarrowia lipolytica)
- Subunit nuim of nadh:ubiquinone oxidoreductase (complex i) (25 kDa, Protein from Candida lipolytica)
- Subunit nb2m of nadh:ubiquinone oxidoreductase (complex i) (6 kDa, Protein from Yarrowia lipolytica)
- Iron/sulfur cluster (351 Da, Ligand)
- Subunit nb5m of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Zinc ion (65 Da, Ligand)
- Subunit nu3m of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Yarrowia lipolytica)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Subunit ni2m of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Candida lipolytica)
- Acyl carrier protein acpm2 of nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Mitochondrial nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)
- Subunit nu4m of nadh:ubiquinone oxidoreductase (complex i) (54 kDa, Protein from Yarrowia lipolytica)
- Subunit nufm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nuhm of nadh:ubiquinone oxidoreductase (complex i) (27 kDa, Protein from Candida lipolytica)
- Subunit ni8m of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Candida lipolytica)
- Subunit nuum of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Flavin mononucleotide (456 Da, Ligand)
- Subunit nunm of nadh:ubiquinone oxidoreductase (complex i) (13 kDa, Protein from Yarrowia lipolytica)
- Subunit nu5m of nadh:ubiquinone oxidoreductase (complex i) (73 kDa, Protein from Yarrowia lipolytica)
- Subunit nucm of nadh:ubiquinone oxidoreductase (complex i) (52 kDa, Protein from Candida lipolytica)
- Subunit nulm of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Subunit nebm of nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Subunit ni9m of nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Subunit nupm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nujm of nadh:ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Yarrowia lipolytica)
- Subunit nuzm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Subunit nukm of nadh:ubiquinone oxidoreductase (complex i) (23 kDa, Protein from Candida lipolytica)
- Subunit nu6m of nadh:ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Yarrowia lipolytica)
- Subunit nipm of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Candida lipolytica)
- Subunit nu2m of nadh:ubiquinone oxidoreductase (complex i) (53 kDa, Protein from Yarrowia lipolytica)
- Subunit numm of nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Candida lipolytica)
- Subunit nubm of nadh:ubiquinone oxidoreductase (complex i) (53 kDa, Protein from Candida lipolytica)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Subunit nesm of nadh:ubiquinone oxidoreductase (complex i) (28 kDa, Protein from Yarrowia lipolytica)
- Subunit nb4m of protein nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Candida lipolytica)
- Subunit nugm of nadh:ubiquinone oxidoreductase (complex i) (32 kDa, Protein from Candida lipolytica)
- Subunit nidm of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Candida lipolytica)
Resolution: 7.6 Å
EM Method: Single-particle
Fitted PDBs: 5my1
Q-score: 0.113
Kohler R, Mooney RA, Mills DJ, Landick R, Cramer P
Science (2017) 356 pp. 194-197 [ Pubmed: 28408604 DOI: doi:10.1126/science.aal3059 ]
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- Translating ribosome (Complex from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- Transcribing rna polymerase (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- In vitro reconstituted e. coli expressome consisting of transcribing rna polymerase and translating ribosome (Complex)
- 30s ribosomal protein s7 (19 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (25 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
CryoEM structure of the membrane pore complex of Pneumolysin at 4.5A
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5ly6
Q-score: 0.259
van Pee K, Neuhaus A, D'Imprima E, Mills DJ, Kuhlbrandt W, Yildiz O
eLife (2017) 6 [ DOI: doi:10.7554/eLife.23644 Pubmed: 28323617 ]
Resolution: 9.1 Å
EM Method: Single-particle
Fitted PDBs: 5luf
Q-score: 0.116
Sousa JS, Mills DJ, Vonck J, Kuhlbrandt W
eLife (2016) 5 [ DOI: doi:10.7554/eLife.21290 Pubmed: 27830641 ]
- Complex i (6 kDa, Protein from Bos taurus)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Bovine)
- Cytochrome c oxidase subunit 7c, mitochondrial (5 kDa, Protein from Bovine)
- Complex i (6 kDa, Protein from Bos taurus)
- Complex i (29 kDa, Protein from Bos taurus)
- Complex i (26 kDa, Protein from Bos taurus)
- Complex i (70 kDa, Protein from Bos taurus)
- Complex i (6 kDa, Protein from Bos taurus)
- Cytochrome b-c1 complex subunit 2, mitochondrial (46 kDa, Protein from Bovine)
- Cytochrome b-c1 complex subunit 6, mitochondrial (9 kDa, Protein from Bovine)
- Heme-a (852 Da, Ligand)
- Cytochrome b (42 kDa, Protein from Bovine)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (7 kDa, Protein from Bovine)
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Bovine)
- Cytochrome c oxidase subunit 5a, mitochondrial (12 kDa, Protein from Bovine)
- Cytochrome c oxidase subunit 2 (26 kDa, Protein from Bovine)
- Cytochrome c oxidase subunit 7a1, mitochondrial (6 kDa, Protein from Bovine)
- Complex i (18 kDa, Protein from Bos taurus)
- Complex i (4 kDa, Protein from Bos taurus)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Complex i (38 kDa, Protein from Bos taurus)
- Complex i (9 kDa, Protein from Bos taurus)
- Complex i (7 kDa, Protein from Bos taurus)
- Complex i (9 kDa, Protein from Bos taurus)
- Cytochrome c oxidase subunit 5b, mitochondrial (10 kDa, Protein from Bovine)
- Cytochrome c1, heme protein, mitochondrial (27 kDa, Protein from Bovine)
- Cytochrome b-c1 complex subunit 1, mitochondrial (49 kDa, Protein from Bovine)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Bovine)
- Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (17 kDa, Protein from Bovine)
- Cytochrome c oxidase subunit 7b, mitochondrial (6 kDa, Protein from Bovine)
- Magnesium ion (24 Da, Ligand)
- Complex i (12 kDa, Protein from Bos taurus)
- Cytochrome b-c1 complex subunit 9 (7 kDa, Protein from Bovine)
- Heme c (618 Da, Ligand)
- Complex i (13 kDa, Protein from Bos taurus)
- Complex i (12 kDa, Protein from Bos taurus)
- Cytochrome c oxidase subunit 6a2, mitochondrial (9 kDa, Protein from Bovine)
- Complex i (46 kDa, Protein from Bos taurus)
- Complex i (6 kDa, Protein from Bos taurus)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Bovine)
- Complex i (32 kDa, Protein from Bos taurus)
- Complex i (25 kDa, Protein from Bos taurus)
- Complex i (35 kDa, Protein from Bos taurus)
- Complex i (13 kDa, Protein from Bos taurus)
- Complex i (13 kDa, Protein from Bos taurus)
- Complex i (28 kDa, Protein from Bos taurus)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (21 kDa, Protein from Bovine)
- Complex i (14 kDa, Protein from Bos taurus)
- Complex i (53 kDa, Protein from Bos taurus)
- Respirasome (1 MDa, Complex from Bos taurus)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Bovine)
- Complex i (51 kDa, Protein from Bos taurus)
- Cytochrome c oxidase subunit 8b, mitochondrial (4 kDa, Protein from Bovine)
- Cytochrome c oxidase subunit 6b1 (10 kDa, Protein from Bovine)
- Complex i (7 kDa, Protein from Bos taurus)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome c oxidase subunit 1 (57 kDa, Protein from Bovine)
- Iron/sulfur cluster (351 Da, Ligand)
Sousa JS, Mills DJ, Vonck J, Kuehlbrandt W
eLife (2016) 5 pp. e21290- [ DOI: doi:10.7554/eLife.21290 Pubmed: 27830641 ]
- Respirasome (1 MDa, Complex from Bos taurus)
Cryo-EM of bovine respiratory supercomplex
Sousa JS, Mills DJ, Vonck J, Kuehlbrandt W
eLife (2016) 5 pp. e21290- [ DOI: doi:10.7554/eLife.21290 Pubmed: 27830641 ]
- Respirasome (1 MDa, Complex from Bos taurus)
Respiratory complex I of Yarrowia lipolytica
D'Imprima E, Mills DJ, Parey K, Brandt U, Kuhlbrandt W, Zickermann V, Vonck J
Biochim Biophys Acta (2016) 1857 pp. 1935-1942 [ DOI: doi:10.1016/j.bbabio.2016.09.014 Pubmed: 27693469 ]
- Nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)
Respiratory complex I of Yarrowia lipolytica with sulfur transferase subunit ST1 bound
D'Imprima E, Mills DJ, Parey K, Brandt U, Kuhlbrandt W, Zickermann V, Vonck J
Biochim Biophys Acta (2016) 1857 pp. 1935-1942 [ DOI: doi:10.1016/j.bbabio.2016.09.014 Pubmed: 27693469 ]
- Nadh:ubiquinone oxidoreductase (1 MDa, Complex from Yarrowia lipolytica)
Electron cryo-microscopy of fatty acid synthase (FAS) from Rhodospiridium toruloides
Fischer M, Rhinow D, Zhu Z, Mills DJ, Zhao ZK, Vonck J, Grininger M
Protein Sci (2015) 24 pp. 987-995 [ Pubmed: 25761671 DOI: doi:10.1002/pro.2678 ]
Electron cryo-microscopy of mitochondrial ATP synthase dimers
Allegretti M, Klusch N, Mills DJ, Vonck J, Kuehlbrandt W, Davies KM
Nature (2015) 521 pp. 237-240 [ Pubmed: 25707805 DOI: doi:10.1038/nature14185 ]
- Polytomella atp-synthase (1 MDa, Sample)
- Mitochondrial f-type atp-synthase (1 MDa, Protein from Polytomella)